UC Davis Molecular Structure Core

The ability to determine the amino acid sequence of a protein was a major advancement in understanding protein structure and function relationships. In the era of biotechnology, sequence analysis still occupies a position of primary importance. Common uses for protein sequence data include design of DNA probes and/or PCR primers, verification of the identity of expressed recombinant proteins, production of synthetic peptide antigens and characterization of post-translational protein modification. In addition, sequence data coupled with a computer search of protein databases has become very important in determining the novelty/identity of any isolated protein.

However, the major barrier to productive microsequencing projects is not the instrumentation but rather the successful purification of proteins/peptides suitable for analysis. Even the most sophisticated, sensitive instrument can not generate useful data from impure and/or inadequate amounts of protein. Unfortunately, most proteins of interest are available in very low abundance. Therefore, sample preparation is the most critical and challenging task in a successful microsequencing project today. The difficulties involved in the purification, storage and recovery of proteins are the major hindrances to the biochemical characterization and biological assay of low abundance proteins.

Molecular Structure Facility
1414 GBSF
451 E. Health Sciences Dr.
University of California, Davis
Davis, CA 95616
USA