Dana Farber Cancer Institute - Flow Cytometry Core

The Flow Cytometry Core Facility provides sterile sorting and analysis of samples. The core houses three high-speed cell sorters with up to 9-color simultaneous detection utilizing multi-laser platforms. Sample populations can be sterile sorted into a variety of tubes or plates. Single cells can also be sterile sorted into individual wells for culturing. Additionally, the cell sorters can sort up to four populations simultaneously. For sample analysis, the lab houses six tabletop analyzers ranging from very simple 3-color detection systems to a high-end instrument with up to 12-color detection utilizing three lasers. It also has a 96-well plate loader for analyzing samples directly from plates. Staff assist researchers with their sorting and analysis needs, including data acquisition, analysis and interpretation, and experiment design, implementation, and troubleshooting. Training classes are offered for researchers who want to independently run a core flow cytometer.

Services

• Cell Sorting and Analysis
• Sterile sorting into tubes or plates
• 4 populations sorted simultaneously
• Automated 96-well plate loader for analysis
• Online Scheduler Reservation System
• Self-run training classes for: FACScan, Luminex (Bio-Plex 200), LSRII, MoFLO-FACSAria-Sorting