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LakePharma
LakePharma is listed as a mid sized business research supplier in the Scientist.com network, with 4 sites in 1 country and 1 service area.
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Sites
4
Countries
1
Services
What this research supplier says it provides, as listed in the Scientist.com network.
Type of company: Mid sized business
Antibodies
- Antibody Conjugation Conjugate an enzyme, biotin, or fluorophore to an antibody.
- Antibody Detection Quantitate, localize and visualize proteins and other antigens.
- Antibody Humanization Increase an antibody's similarity to a variant produced naturally in humans.
- Antibody Isotype Switching Modify antibody isotypes from any species to any human IgG isotype.
- Antibody Products Purchase antibody, antibody pair kit or ELISA kit.
- Antibody Protein De Novo Sequencing Sequence antibody proteins using multiple enzyme digestion and high-resolution LC-MS/MS.
Show all 20 in Antibodies
- Antibody Purification Purify multiple antibody types including IgG, IgM, IgA and IgY.
- Antibody Sequencing Sequence cDNA encoding variable heavy (VH) and variable light (VL) domains of a hybridoma cell line.
- Antibody Services Antibodies have become an essential tool in most life science and drug discovery research projects. Both monoclonal and polyclo...
- Antibody-Drug Conjugation Improve therapeutic properties of an antibody or drug by joining them together.
- Biotin-Antibody Conjugation Biotinylate an antibody for improved detection.
- Direct Antibody Labeling Conjugate a primary antibody to a label for direct antigen detection.
- Epitope Mapping Map the antibody-antigen binding region to define protein-protein interaction sites.
- Hybridoma Sequencing/Antibody Sequencing Determine the sequences of monoclonal antibodies.
- In Vitro Monoclonal Antibody Production Generate monoclonal antibodies in vitro using cultured antibody-secreting hybridoma cell lines.
- OmniRat Transgenic Animal Platform Produce humanized antibodies using a transgenic rat platform.
- Phosphospecific Antibodies Create antibodies to detect phosphorylated protein targets.
- Polyclonal/Monoclonal Antibody Production Generate polyclonal or monoclonal antibodies in a range of animal species, over-express antibodies in cultured cells.
- Rabbit Monoclonal Antibody Development Develop monoclonal antibodies using rabbit as the host animal.
- Single Chain Variable Fragment (scFv) Create an antigen-specific antibody derivative using a linker to join a heavy and a light variable chain.
Biomarkers
- Cell Migration Assays Measure chemotaxis, haptotaxis, transmigration and wound-healing.
- Cell Viability Measure changes in cell number, cell division, DNA content or a cell metabolism marker such as ATP levels.
- Cytokine Release Assay Use in vivo or in vitro assays to detect unregulated or excessive release of pro-inflammatory cytokines.
- Flow Cytometry Identify fluorescently-labeled cells or physically separate cells from a larger population using biomarkers.
- Immunoassays Create antibody-based assays for the identification and quantification of specific antigens.
- Immunophenotyping Separate cell types based on their expression of biomarkers or based on their individual and unique expression patterns.
Show all 7 in Biomarkers
- Lipid Kinase Assays Use biochemical, cell-based or in vivo assays to measure lipid kinase activity.
Show all 106 services
Cell Culture and Characterization
- Bacterial Cell Culture Culture and Grow bacterial cells.
- Cell Culture Culture primary or immortalized cell lines.
- Cell Line Construction Create new recombinant cell lines to exact specifications.
- Cell Services Most drug research projects routinely use a variety of cell research services including cell line construction, analysis of cells.
- Insect Tissue Culture Grow insect cells.
- Recombinant Technology Manipulate genotypes to introduce or modify the expression of specific cell products or physical traits.
Show all 7 in Cell Culture and Characterization
- Yeast Cell Culture Grow yeast strains or species.
Chemical and Compound Analysis
- Differential Scanning Calorimetry Elucidate the thermodynamics associated with conformational transitions.
- Endotoxin Testing Use standard assays like LAL (Limulus Amebocyte Lysate), rFC (Recombinant Factor C), or MAT (Monocyte Activation Test) to detect and quantify the level of endotoxins in a sample
- GLP Large Molecule Bioanalysis Detect and analyze the pharmacokinetic profile of large biomolecules.
- Immunoaffinity Chromatography Use antibody-antigen interactions to isolate and concentrate analytes.
- Isothermal Titration Calorimetry An ITC assay will be established and optimised for the affinity measurement of test substance to target molecule.
- Protein Melting Point Analysis Assess protein stability by Differential Scanning Calorimetry (DSC) or Fluorimetry (Thermofluor) methods.
Show all 7 in Chemical and Compound Analysis
- Size Exclusion Chromatography Separate and identify analytes based on their molecular size.
Chemistry, Manufacturing and Controls (CMC)
- Analytical Development and Validation Request services related to Analytical Development and Validation
- Chemistry, Manufacturing and Controls (CMC) Request Chemistry, Manufacturing and Controls services
- Drug Product Stability Use accelerated stress conditions to determine degradation pathways and identify manufacturing issues.
- GMP Synthesis Synthesize APIs including small molecules, formulations and protein-based drugs under GMP compliance.
- Thermal Analysis Characterize compounds based on calorimetry and thermodynamic data.
Discovery Chemistry
- LCMS Analysis Physically separate substances using liquid chromatography and mass spectrometry.
DNA & RNA
- cDNA cloning & subcloning Move cDNA fragments between different cloning vectors.
- Cloning/Subcloning Clone or Subclone nucleotide sequence or gene.
- CRISPR Using advanced CRISPR technology, add or delete base pairs at a specifically targeted DNA loci.
- Digital Polymerase Chain Reaction Obtain improved absolute target quantification and rare allele detection compared to conventional qPCR.
- DNA Extraction Extract DNA from an original source.
- DNA Mutagenesis Introduce insertions, deletions and substitutions into DNA clones.
Show all 16 in DNA & RNA
- DNA Sequencing Determine the nucleotide sequence of DNA samples.
- DNA Services From whole genome sequencing to large scale DNA and oligonucletotide synthesis, DNA services have become routine tools in drug ...
- DNA Synthesis Produce DNA fragments for microarrays, PCR, cloning and expression studies.
- Gene/Oligo Synthesis Synthesize oligonucleotide or gene for microarrays, RT-PCR and PCR cloning.
- Genomic DNA cloning Clone and propagate genomic DNA inserts of 5-50kb.
- Genomic DNA Purification Isolate genomic DNA from cells or tissue samples.
- miRNA sequencing Identify miRNAs by Next Generation Sequencing and bioinformatic analysis.
- Plasmid DNA Preparation and Extraction Prepare plasmid DNA starting from E. coli cell pellets, transformed colonies or DNA samples.
- qPCR Analysis Measure the accumulation of PCR products in real time.
- Quantitative RT PCR Use quantitative reverse transcription polymerase chain reaction to measure absolute and relative mRNA abundance.
Human Biological Samples
- Master Cell Banking Characterize, expand, cryopreserve, store and track cell samples.
In Vitro Assays
- Apoptosis Assays Detect apoptotic events that lead to programmed cell death.
- Apoptotic Cell Staining Detect apoptotic cells in culture.
- Assay Development Construct or create new assays or modify existing ones.
- Cell Based Assays Apply compounds to cells and test for cellular phenotypes and effects on gene expression
- Cell Based Assays Consulting Expert advice to evaluate the functions of specific chemicals based on their constructions and systems.
- Cell Proliferation Measure cell number, cell viability and cell division.
Show all 8 in In Vitro Assays
- Luciferase Reporter Assay Evaluate promoter activity by measuring light output from the luciferase enzyme.
- Viral Clearance Assays Measure the capacity to remove or inactivate infectious agents during the manufacturing process.
In Vitro Pharmacology
- High Throughput Screening Conduct millions of chemical, genetic or pharmacological tests in a short period of time.
- In Vitro Enzyme Activity Assay Development Develop new in vitro enzyme activity assays or modify existing ones.
- Kinase Assays Measure activity of over 500 kinases in biochemical or cell-based assays.
- Phosphatase Assays Use biochemical, cell-based or in vitro assays to measure phosphatase activity.
- Protein/Enzyme Targets For requesting Protein/Enzyme Targets
In Vitro Toxicology
- ADCC and CDC Assay Services Measure toxicity of antibodies.
- Cytotoxicity Test for effects on cell viability and proliferation
Protein Sciences
- Adeno Associated Virus (AAV) Production Clone into AAV expression vectors and produce high titer viruses.
- Antibody-Antigen Affinity Measurement Measure the binding kinetics of an antibody–antigen interaction.
- Bacterial Expression Produce milligram, gram and kilogram amounts of proteins.
- Carbohydrate Isolation Obtain pure monosaccharides, pure polysaccharides or related derivatives from samples.
- Carbohydrate Isolation Protocol Development Design and optimize protocols for carbohydrate isolation.
- ELISA Detect the presence of antigens recognized by antibodies or find antibodies that recognize an antigen.
Show all 26 in Protein Sciences
- Fluorescence Polarization Investigate receptor-ligand interactions, protein-DNA interactions, proteolysis and membrane fluidity.
- Fluorophore Protein Conjugation Attach a fluorescent probe to a protein.
- Fusion Proteins Use recombinant DNA technology to create and express proteins derived from more than one gene.
- Insect Expression Use baculovirus infected cells or non-lytic cell systems to produce glycosylated proteins.
- Large Scale Protein Production Produce large amounts of protein using fermentors, wave mixers, bioreactors, and large-scale purification workflows.
- Mammalian Expression Express high yields of recombinant proteins, antibodies, virus, viral-subunit proteins and gene-therapy vectors.
- N-terminal Sequencing Determine the amino acid sequence of an intact protein from its N-terminus.
- Phosphorylation Analysis Identify phosphorylation sites in target proteins.
- Protein Conjugation Create tagged monoclonals, polyclonal and recombinant proteins.
- Protein Expression Produce recombinant proteins in prokaryotic or eukaryotic systems.
- Protein Post-translational Modification Analysis Measure glycosylation, phosphorylation, sulfation, ubiquitination, methylation and acetylation.
- Protein Production Produce recombinant protein molecules in prokaryotic or eukaryotic systems.
- Protein Purification Isolate native or recombinant proteins from a sample.
- Protein Sequencing Determine the amino acid sequence of protein or peptide.
- Protein Services Consulting Expert advice to evaluate and analyze protein function and expression.
- Qualitative MS Perform direct identification of molecules based on mass-to-charge ratio and fragmentation patterns.
- Quantitative MS Quantify small and large molecules in the pg/ml range
- Recombinant Proteins Use recombinant DNA to produce proteins in vivo or in vitro.
- Virus Production Produce high titer viruses, viral expression vectors and vaccines.
- Yeast Expression Amplify eukaryotic proteins that contain post translational modifications.
Other services
- New Request
Service areas:
Biology
Where it operates
Sites by country, with the cities in each.
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United States 4 sites
About this supplier
In the supplier's own words, as listed in the Scientist.com network.
LakePharma is a leading contract research organization (CRO) specializing in antibody and protein engineering, cell line development, and protein production. Services can be fully integrated, creating a “soup to nuts” solution for all of a customer’s molecular biology, cell line, and protein needs. Located in the heart of the San Francisco Bay Area, LakePharma has developed numerous in-house technology platforms to provide cost-effective and high quality services to its customers. The production processes are SOP-based and high-throughput, and have been streamlined to be exceptionally...
Show the full description
LakePharma is a leading contract research organization (CRO) specializing in antibody and protein engineering, cell line development, and protein production. Services can be fully integrated, creating a “soup to nuts” solution for all of a customer’s molecular biology, cell line, and protein needs.
Located in the heart of the San Francisco Bay Area, LakePharma has developed numerous in-house technology platforms to provide cost-effective and high quality services to its customers. The production processes are SOP-based and high-throughput, and have been streamlined to be exceptionally efficient on both a small and large scale. Each project is actively managed by a dedicated study director to ensure timely completion with superior results and transparent communication. Clients have real-time access to their project data and can view and receive status updates through LakePharma’s secure cloud-based Datasystems. LakePharma follows the principles of Good Laboratory Practices (GLP) to ensure the quality, reliability and integrity of study data. Upon request, services can also be performed in full compliance with FDA documentation requirements for submissions for nonclinical laboratory studies. All services are provided solely on a fee-for-service basis and are royalty-free.
We provide services in:
- Cloning molecular, antibody variable region, gene synthesis
- Cell line development - stable cell line expressing single gene, high expressor
- Fc fusion protein DNA construction
- Endotoxin-free plasmid DNA production
- Antibody services - optimization, humanization, recombinant construction, region cloning
- Website
- www.lakepharma.com
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