University of Siena - PCR Research and Diagnostic Core

Real-time PCR Research and Diagnostics Core Facility is a DBE laboratory offering Real-time PCR research including but not limited to human. The laboratory was created to provide researchers with flexible services to detect gene expression both using relative or absolute quantification methods. The facility also provides expertise in the analysis of nucleic acids.

The Core Facility consists of twor fully equipped molecular biology laboratories for sample preparation, extraction, Real-time PCR assay generation, storage, and analysis. The Facility is dedicated to providing diagnostic services for the academic community and private companies by combining powerful last generation Bio-Rad CFX equipment with several years of PCR experience.

We also offer advice on how to apply this technology to your research needs, including the different chemistries and reagents/consumables available, primer and probe design, optimization of assays, quantitation methods, and provide training in the use of the instruments and data analysis.