University of Cambridge - Proteomics Core

The facility is equipped with state-of-the-art instrumentation for CI researchers requiring access to proteomics technology and expertise.

The Proteomics core facility provides help in designing experimental strategies and implements and validates previously developed proteomic workflows to profile proteins from diverse biological samples. We also aim to modify or develop entirely new methods and assays when warranted. In addition, we have bioinformatics support for data management and analysis as well as software development.

The facility has already been well equipped with state-of-the-art analytical instrumentation for proteomic studies, including the latest Orbitrap mass spectrometers: an LTQ Velos Orbitrap (Thermo), which has been configured to a Dionex Ultimate 3000 RSLC nanoHPLC system (Figure 1), and a newly acquired Q Exactive Orbitrap. The mass spectrometers are supported by off-line chromatography platforms: two Dionex Ultimate 3000 capHPLC systems for multidimensional chromatography at the protein and peptide level. These are supported by 1D and 2D gel electrophoresis systems as well as a GE Healthcare Typhoon Trio+ imager available in the equipment park run by Jane Gray. Data analysis is supported by an array of bioinformatics and statistical analysis tools.