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Bilkent University - Hybridoma Lab Core
Bilkent University - Hybridoma Lab Core is listed as an academic core research supplier in the Scientist.com network, with 1 site in 1 country.
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Type of company: Academic core
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Turkiye 1 site
About this supplier
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Monoclonal antibodies are valuable tools in cancer research. Besides, these reagents can be used for diagnostic and prognostic purposes in clinicopathological studies of human tumors. Hybridoma core facility has the expertise to develop monoclonal antibodies by standard cell fusion technique. BALB/c mice from “Rodent Animal House” are immunized with antigens (mostly home-made recombinant proteins) of interest and immunization efficiency is assessed.by indirect ELISA in serially diluted mice sera. The same assessment method is also used for screening of hybridoma clones. Positive clones are...
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Monoclonal antibodies are valuable tools in cancer research. Besides, these reagents can be used for diagnostic and prognostic purposes in clinicopathological studies of human tumors. Hybridoma core facility has the expertise to develop monoclonal antibodies by standard cell fusion technique. BALB/c mice from “Rodent Animal House” are immunized with antigens (mostly home-made recombinant proteins) of interest and immunization efficiency is assessed.by indirect ELISA in serially diluted mice sera. The same assessment method is also used for screening of hybridoma clones. Positive clones are subcloned by either single cell cloning or limited dilution. Hybridomas are expanded in cell culture or alternatively ascites are induced in nude mice. Monoclonal antibodies are purifed in our FPLC facility through protein A or G affinity columns. So far, our Unit produced several monoclonal antibodies including:
- Anti-SIP1 (Smad-inteacting protein 1): clones 1C6, 6E5
- Anti-FLASH (Flice-associated huge protein): clone 1A4
- Anti-ERBB2: clones BH1, BH2, BH5, BH6, BH7
- Anti-BETA-CATENIN: clones 4C9, 9E10
- Anti-PLEXIN C1: clone 2H9
- Anti-TP53: clone 9E4
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