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University of Southampton - Protein Core
University of Southampton - Protein Core is listed as an academic core research supplier in the Scientist.com network, with 1 site in 1 country.
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Type of company: Academic core
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United Kingdom 1 site
About this supplier
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The Protein Core Facility offers services for the production of recombinant proteins for the Cancer Science Division. It is located on level C in the Somers Building. The PECF is aiming to help your research projects in several ways: Consulting for your own projects or to start a project with us Protein expression in bacteria Protein purification The projects can be started at any stage: Cloning of the cDNA in the appropriate bacterial expression vector Induction of the protein, including tests for expression levels and solubility Purification of the recombinant protein by chromatography...
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The Protein Core Facility offers services for the production of recombinant proteins for the Cancer Science Division. It is located on level C in the Somers Building.
The PECF is aiming to help your research projects in several ways:
- Consulting for your own projects or to start a project with us
- Protein expression in bacteria
- Protein purification
The projects can be started at any stage:
- Cloning of the cDNA in the appropriate bacterial expression vector
- Induction of the protein, including tests for expression levels and solubility
- Purification of the recombinant protein by chromatography
Cloning
We routinely use the following bacterial expression vectors:
- pET for the addition of a small His tag
- pGEX for the fusion of the protein to GST
- pMAL for the fusion of the protein to MBP to increase the solubility of some proteins
We welcome any suggestions about vectors you have used and found useful.
Protein expression
The protein expression is carried out in BL21 bacteria strains. For the expression of mammalian proteins, we prefer the use of the strain BL21 CodonPlus which carries extra copies of tRNA genes encoding rarer bacterial codons.
Protein Purification
The protein purifications are performed using two FPLC AKTA Prime systems. A range of columns are available for:
- affinity purification (Nickel for His-tagged proteins, glutathione sepharose for GST-fused proteins and amylose for MBP-fused proteins)
- ion exchange chromatography (Q-sepharose and SP-sepharose)
- gel filtration (size exclusion)
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