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Ramón A Espinoza-Lewis
Ramón A Espinoza-Lewis is listed as a small consultant research supplier in the Scientist.com network, with 1 site in 1 country.
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Sites
1
Countries
1
Services
What this research supplier says it provides, as listed in the Scientist.com network.
Type of company: Small consultant
Cell Culture and Characterization
- Lipofection Transfection of DNA/RNA using lipofection reagents to create lipoplexes.
Chemistry, Manufacturing and Controls (CMC)
- GMP Synthesis Synthesize APIs including small molecules, formulations and protein-based drugs under GMP compliance.
DNA & RNA
- Amplicon 16s rRNA Sequencing Identify and compare bacteria present within a given sample.
- Amplified Fragment Length Polymorphism Detection Detect DNA polymorphisms based upon restriction enzyme digestion and PCR.
- cDNA cloning & subcloning Move cDNA fragments between different cloning vectors.
- Cloning/Subcloning Clone or Subclone nucleotide sequence or gene.
- De Novo Genome Sequencing Determine the DNA sequence of uncharacterized genomes.
- Digital Polymerase Chain Reaction Obtain improved absolute target quantification and rare allele detection compared to conventional qPCR.
Show all 36 in DNA & RNA
- DNA Mutagenesis Introduce insertions, deletions and substitutions into DNA clones.
- DNA Sequencing Determine the nucleotide sequence of DNA samples.
- DNA Synthesis Produce DNA fragments for microarrays, PCR, cloning and expression studies.
- DNA/RNA Extraction & Purification DNA/RNA Extraction & Purification.
- Gene/Oligo Synthesis Synthesize oligonucleotide or gene for microarrays, RT-PCR and PCR cloning.
- Genomic DNA cloning Clone and propagate genomic DNA inserts of 5-50kb.
- Genomic DNA Purification Isolate genomic DNA from cells or tissue samples.
- Genotyping Examine a DNA sequence to identify specific genetic varients .
- miRNA sequencing Identify miRNAs by Next Generation Sequencing and bioinformatic analysis.
- NGS: Next Generation Sequencing Sequence millions of template DNA molecules in parallel.
- Oligo Synthesis Produce oligonucleotides for microarrays, RT-PCR and PCR cloning.
- Poly (A) tail Length Profiling (PAL-Seq) Conduct high-throughput sequencing of individual mRNA poly (A) tails.
- qPCR Analysis Measure the accumulation of PCR products in real time.
- Quantitative RT PCR Use quantitative reverse transcription polymerase chain reaction to measure absolute and relative mRNA abundance.
- Recombinant Nucleosomes Use as substrates in histone methyltransferase or acetyltransferase assays.
- Restriction Fragment Length Polymorphism Identification Uses gel electrophoresis to identify differences in restriction fragment lengths produced by enzymatic digestion of DNA.
- RNA Amplification Produce RNA from pre-clinical and clinical samples for use in gene expression studies.
- RNA Extraction Isolate total RNA or mRNA from cells or tissues
- RNA Interference Use small sequences of RNA that correspond to the targeted gene product to inhibit mRNA translation.
- RNA oligo synthesis Design and synthesize miRNAs, siRNAs and other non coding RNAs
- RNA Profiling Quantify the expression levels of mRNA transcripts, enabling the determination of gene expression patterns.
- RNA Sequencing Use Next Generation Sequencing techniques to Identify RNA transcripts, examine differential expression patterns and analyze splice variants.
- RNA Sequencing/Resequencing Identify RNA transcripts, examine differential expression patterns and analyze splice variants.
- RNA Services Whether you’re isolating total RNA for whole genome expression analysis by microarray or setting up high content screening by s...
- RNAscope In Situ Assays Profile single-cell gene expression in situ.
- Sanger Sequencing Sequence single strand DNA fragments using DNA Polymerase.
- siRNA Synthesis Produce high quality siRNAs for gene silencing studies.
- Target Gene Expression Measure expression of a target gene at the protein, RNA and DNA level.
- Whole Genome Amplification (WGA) Amplify an entire genome from a single cell.
- Whole Genome Sequencing Determine the entire genetic make-up of an organism.
Drug Development
- Consulting Services Work with consultants who provide expert advice in various areas of research and technology.
- Target Validation Consulting Help to demonstrate that modification of target activity has potential therapeutic benefits.
Where it operates
Sites by country, with the cities in each.
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Spain 1 site
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