University of Colorado - Boulder BioFrontier Institute: Cell Culture Facility

The BL-2 certified cell culture facility in JSCBB is shared by all Biochemistry investigators and is equipped with twelve containment hoods. Six of these are for general use, two hoods are specific for primary cell culture work and two are located in a separate room for use with virus. In addition, there is a horizontal, laminar flow hood for use with large volume cultures and a restricted hood for Salmonella invasion studies of mammalian cell hosts. There are thirteen CO2 incubators as well as an incubator and shaker for working with insect cell cultures. The facility has the ability to do experiments requiring hypoxic conditions. It also has two automated liquid nitrogen storage units and an inverted microscope with both light and fluorescence sources. The facility is supervised by Theresa Nahreini who trains all users in sterile techniques and oversees daily operations of media preparation, mycoplasma testing and maintenance of cell lines.