Johns Hopkins University Bayview Flow Cytometry Core Facility

The Bayview Flow Cytometry Core Facility was established in 2008 to serve the biomedical research needs of Johns Hopkins Investigators located on the Bayview Campus. The core is managed by Joe Chrest who brings over 25 years of flow cytometry experience to investigators who need to use this technology to address problems in modern biomedical science. The core is uniquely embedded among research groups interested in problems related to immune based human diseases and relevant animal models and promotes an interactive and collaborative approach. The facility instrumentation has the capability of performing simple and complex multiparamter (up to 14 fluorochromes) analysis of cell subpopulations and high speed sorting.

We are presently equipped with a four color FACSCalibur (BD BioSciences), a special order FACSAria cell sorter (BD BioSciences) with 14-fluorescence parameter capability, and 2 analysis workstations for off-line data analysis. The FACSCalibur and FACSAria are maintained using the highest standards of quality control and are available for analysis to certified and trained users. Cell sorting is performed by flow core staff only. Our staff has over forty years of combined flow cytometry and cell sorting experience and offers free consultation services.